Research Articles
Troubleshooting STAT Dimerization Assays: A Comprehensive Guide for Cellular Systems Research
This article provides researchers, scientists, and drug development professionals with a comprehensive framework for understanding, performing, and troubleshooting STAT protein dimerization assays in cellular systems.
Optimizing SH2 Domain Structural Models for Enhanced Virtual Screening in Drug Discovery
This article provides a comprehensive guide for researchers and drug development professionals on optimizing SH2 domain structural models to improve the success rate of virtual screening.
Targeting the Distinction: Strategies for Achieving Selectivity Between STAT and Src-Family SH2 Domains in Drug Discovery
This article provides a comprehensive analysis of the strategies and challenges in achieving high selectivity between the SH2 domains of STAT and Src-family kinases (SFKs), a critical goal for developing...
Overcoming Low Affinity in STAT SH2 Domain Inhibitor Development: Strategies for Selective and Potent Therapeutics
The development of high-affinity inhibitors targeting the STAT-SH2 domain represents a formidable challenge in drug discovery, primarily due to the conserved nature of the phosphotyrosine-binding pocket and the poor pharmacokinetic...
Intracellular SH2-Binding Proteins: Engineered Tools to Perturb and Decipher Cell Signaling
This article explores the cutting-edge strategy of using intracellularly expressed SH2-binding proteins as targeted tools to disrupt phosphotyrosine signaling networks.
Evolution and Classification of SH2 Domains: Phylogenetic Analysis, Methodologies, and Clinical Implications
This article provides a comprehensive resource for researchers and drug development professionals on the phylogenetic analysis and classification of Src Homology 2 (SH2) domains.
Targeting STAT SH2 Domains: A Computational Guide to pY and pY+3 Pocket Docking for Drug Discovery
This article provides a comprehensive resource for researchers and drug development professionals on employing molecular docking to target the phosphotyrosine (pY) and pY+3 pockets of STAT SH2 domains.
Competitive Binding Assays for STAT SH2 Domain Inhibitor Screening: A Guide for Drug Discovery
This article provides a comprehensive guide for researchers and drug development professionals on utilizing competitive binding assays to screen for inhibitors targeting the Src Homology 2 (SH2) domains of STAT...
Targeting SFK SH2 Domains with Monobodies: A High-Precision Strategy for Dissecting Signaling and Inhibiting Oncogenesis
Src family kinases (SFKs) are critical signaling proteins whose functions are heavily dependent on their Src Homology 2 (SH2) domains.
Yeast Surface Display for SH2 Domain Binders: A Comprehensive Guide from Library Selection to Clinical Applications
This article provides a comprehensive methodological and practical guide for researchers and drug development professionals on employing yeast surface display (YSD) for selecting and engineering high-affinity binding proteins against Src...