Research Articles
Bridging the Gap: A Comprehensive Guide to Biochemical vs. Cellular IC50 Values in Drug Discovery
This article provides a comprehensive analysis of the critical differences between IC50 values obtained from biochemical and cellular assays, a common challenge in drug discovery.
Specificity and Selectivity in Analytical Method Validation: A Guide to ICH Q2(R2) Compliance
This article provides a comprehensive guide for researchers, scientists, and drug development professionals on validating the specificity and selectivity of analytical methods.
Spectrophotometry vs. UFLC-DAD: A Comprehensive Guide for Method Selection in Pharmaceutical Analysis
This article provides a systematic comparison between spectrophotometric and Ultra-Fast Liquid Chromatography with Diode Array Detection (UFLC-DAD) methods for pharmaceutical analysis.
Comparative Screening for STAT-Specific Inhibitors: Strategies, Challenges, and Clinical Pipeline Insights
This article provides a comprehensive analysis of comparative screening methodologies for developing specific Signal Transducer and Activator of Transcription (STAT) inhibitors.
Beyond the Test Tube: Bridging the Gap Between In Vitro and Cellular Enzyme Kinetics in Crowded Environments
This article addresses the critical challenge of reconciling discrepancies in enzyme kinetic data obtained from simplified in vitro assays versus complex cellular environments.
Beyond PBS: Optimizing K+ and Na+ Levels for Physiologically Relevant Intracellular Mimicry in Biomedical Research
This article provides a comprehensive guide for researchers and drug development professionals on replicating the intracellular ionic environment in vitro.
Bridging the Divide: A Strategic Guide to Managing Intracellular vs. Extracellular Physicochemical Conditions in Biomedical Research
This article provides a comprehensive guide for researchers and drug development professionals on the critical, yet often overlooked, differences between intracellular and extracellular physicochemical environments.
Beyond Dilute Solutions: A Practical Guide to Correcting for Molecular Crowding in Protein-Ligand Binding Assays
This article provides a comprehensive resource for researchers and drug development professionals grappling with the significant effects of molecular crowding on protein-ligand interactions.
Bridging the Assay Gap: A Strategic Guide to Optimizing Cytoplasm-Mimicking Buffers for Predictive Drug Discovery
This article addresses a critical challenge in biomedical research: the persistent discrepancy between biochemical assay (BcA) and cell-based assay (CBA) results, which often delays drug development.
Bridging the Gap: A Strategic Guide to Resolving Discrepancies Between Biochemical and Cellular Assay Results
Inconsistencies between biochemical assay (BcA) and cell-based assay (CBA) data are a major hurdle in drug discovery, often leading to delayed projects and misinterpreted structure-activity relationships.