Research Articles
Weak Western Blot Bands? A Scientist's Complete Troubleshooting Guide (2025)
Weak or absent protein bands are a common frustration in Western blotting that can stem from issues at any stage of the process.
A Complete Guide to Eliminating Streaking in 2D Gel Electrophoresis
This article provides a comprehensive guide for researchers and drug development professionals seeking to resolve the pervasive issue of streaking in two-dimensional polyacrylamide gel electrophoresis (2D-PAGE).
Maximizing Protein Yield: Advanced Strategies to Overcome Low Recovery from Polyacrylamide Gels
This article provides a comprehensive guide for researchers and drug development professionals struggling with low protein recovery from polyacrylamide gels.
Preserving Protein Truth: A Strategic Guide to Preventing Denaturation in Native PAGE for Functional Analysis
This article provides a comprehensive guide for researchers and drug development professionals on preventing protein denaturation during Native Polyacrylamide Gel Electrophoresis (PAGE).
Advanced Strategies for High Molecular Weight Protein Resolution: From Western Blotting to Structural Analysis
This article provides a comprehensive guide for researchers and drug development professionals seeking to overcome the significant challenges associated with analyzing high molecular weight (HMW) proteins.
Solving Smiling Bands in PAGE: A Complete Troubleshooting Guide for Reliable Protein and Nucleic Acid Analysis
This article provides a comprehensive guide for researchers and drug development professionals on diagnosing, troubleshooting, and preventing the 'smiling bands' phenomenon in polyacrylamide gel electrophoresis (PAGE).
SDS-PAGE Gel Percentage Selection: A Complete Guide to Optimizing Protein Separation by Molecular Weight
This article provides a comprehensive guide for researchers and drug development professionals on selecting the optimal polyacrylamide gel percentage for SDS-PAGE to achieve high-resolution protein separation.
Extracting Active Proteins from Native Gels: A Guide to Protocols, Applications, and Troubleshooting for Biomedical Research
This article provides a comprehensive guide for researchers and drug development professionals on the extraction of active proteins from native polyacrylamide gels.
Comprehensive 2D-PAGE Guide: Integrating Native and SDS-PAGE for Advanced Protein Characterization
This article provides researchers, scientists, and drug development professionals with a comprehensive framework for implementing two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) that sequentially combines Native PAGE and SDS-PAGE.
Mastering SDS-PAGE for Western Blotting: A Complete Guide to Optimal Sample Preparation and Separation
This comprehensive guide details the critical role of SDS-PAGE in Western blotting sample preparation for researchers and drug development professionals.